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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in advertising and marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two added splice variants were being documented but detailed nucleotide sequence data is not yet offered.
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Blocking peptides are peptides that bind exclusively on the goal antibody and block antibody binding. These peptide normally incorporates the epitope recognized by the antibody.
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This manual outlines even more doable optimization of mobile and immunoassay parameters to be certain the best possible success are...
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This guideline outlines even more feasible optimization of mobile and immunoassay parameters to ensure the absolute best final results are...
Antibodies bound to the blocking peptide no longer bind into the epitope to the target protein. By evaluating the staining from your blocked antibody compared to the antibody alone, you can see which staining is particular.
Target Information and facts Cyclin E belongs for the highly conserved cyclin family, whose associates are characterised by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation patterns which lead into the temporal coordination of every mitotic celebration. Cyclin E forms a fancy with and capabilities to be a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates for the G1-S section boundary and is degraded as cells progress by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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